Aligning NGS Data in Sequencher 5 with GSNAP and Tablet
Sequencher 5.1 Demo
"Whether it has been for building contigs in the search for novel causative mutations, verifying proper vector construction, or simply aligning large numbers of samples for typing of a single SNP, it has been an invaluable tool. With the advent of next generation sequencing technologies, it becomes even more necessary to have trustworthy, user-friendly software. "
Rob Loechel, Chief Scientific Officer, Vetgen, L.L.C.
"Sequencher is not just the best sequence analysis program I have used; it is one of the best pieces of software I have used. Period. It is intuitive; it does exactly what I want it to do in pretty much the way I would expect it to work. "
Wilson Clements, Ph.D., University of California, San Diego
"I just wanted to let you know how much the modifications to Sequencher have assisted in our data flow. The time saving has been amazing. Assembling our data has taken third the time it usually does, and has allowed for the smoothest assembly of our data for a vaccine selection that I can remember. Additionally noteworthy as we have produced more data for this selection than ever before. Please let your programmers know that the work that they have done has truly helped and thank them for their efforts. "
An Associate Research Fellow, US Centers for Disease Control and Prevention
"The most popular request I receive in the DNA core lab is: find the good clone among this group of 'candidate' minipreps. Alignment of ABI data files against a 'reference' sequence (template file) quickly eliminates defectives by showing not only base changes but the consequences to the protein translation. The second most popular request is: validate this new maxiprep so it can be given to ... or used for ... or expressed in ... Rapid assembly and editing of total plasmid sequence and then comparison to the reference file on record is all done in Sequencher. The third most popular request is: compare this library of (protein improvement) mutants and tell me which ones have protein changes. This is done by alignment in Sequencher and then formatting the Summary view to display the resulting changes in protein translation. For my own projects, I use Sequencher as a very general multiple-alignment sequence editor. Most recently, I've been using it to make comparisons of genes retrieved from the databases for cross-species cDNA cloning. Showing regions of homology at the protein level and then having the colinear DNA sequence presented in the same view (Summary window) helps find regions suitable for primer design. Turning on the Matching Bases/Residues as Dashes quickly shows regions of homology and divergence. I design primers to either or both depending on the project. "
Dean Regier, Research Scientist DNA core laboratories
" Sequencher 4.10.1 contains a new feature that keeps track of protein and cDNA numbering in DNA assemblies. This ability greatly simplifies the task of comparing your experimental data to a genomic DNA reference when looking for mutations or other changes. This feature alone reinforces Sequencher as one of the premier tools for sequence analysis. "
David Adams, M.D Ph.D Clinical Investigator and Sequencher 4.10.1 beta tester
"Sequencher, from Gene Codes of Ann Arbor, Mich., is possibly today's most popular software for DNA Sequencing. "
Steven D. Buckingham, Sequence Analysis Software Update, The Scientist, December 20, 2004
"After using Sequencher for years as a grad student, I was forced to switch to MacVector as a postdoc in a new lab. Sequencher is so much easier to use than MacVector and has such a superior interface that I used the demo version of Sequencher for months rather than the lab copy of MacVector. Sequencher feels as though it is written by biologists that can write software, rather than programmers attempting to write biological software. Any life scientist who has spent time pulling their hair out over non-intuitive software will greatly appreciate this distinction. "
Dave Parker, University Of Michigan Cell And Developmental Biology
"I am very satisfied with how sequencher handles heterozygous calls in autosomal sequencing and potential heteroplasmies in mitochondrial resequencing. I found the forensics addon package for the general forensics community to be very helpful! "
Eric Wang, Ph.D Statistical Geneticist, Algorithm Development and Data Analysis Affymetrix, Inc.