A new Macintosh version of Sequencher fully compatible with Apple's OS 10.7 (Lion) will begin beta-testing in September. Keep checking our website or follow us on Facebook, LinkedIn and Twitter for updates on the final release schedule.
Gene Codes Corporation announces the release of Sequencher 5.0. It incorporates new DNA sequence data alignment algorithms with SNP and methylation analysis for next-generation DNA sequence data. Performance for assembly and alignment of sequences and contigs is hundreds of times faster. New alignment functions and database searching features make getting reliable results from both capillary electrophoresis and next-generation data faster than ever.
"I just wanted to let you know how much the modifications to Sequencher have assisted in our data flow. The time saving has been amazing. Assembling our data has taken third the time it usually does, and has allowed for the smoothest assembly of our data for a vaccine selection that I can remember. Additionally noteworthy as we have produced more data for this selection than ever before. Please let your programmers know that the work that they have done has truly helped and thank them for their efforts.
An Associate Research Fellow, US Centers for Disease Control and Prevention
"The most popular request I receive in the DNA core lab is: find the good clone among this group of 'candidate' minipreps. Alignment of ABI data files against a 'reference' sequence (template file) quickly eliminates defectives by showing not only base changes but the consequences to the protein translation.
The second most popular request is: validate this new maxiprep so it can be given to ... or used for ... or expressed in ... Rapid assembly and editing of total plasmid sequence and then comparison to the reference file on record is all done in Sequencher.
The third most popular request is: compare this library of (protein improvement) mutants and tell me which ones have protein changes. This is done by alignment in Sequencher and then formatting the Summary view to display the resulting changes in protein translation.
For my own projects, I use Sequencher as a very general multiple-alignment sequence editor. Most recently, I've been using it to make comparisons of genes retrieved from the databases for cross-species cDNA cloning. Showing regions of homology at the protein level and then having the colinear DNA sequence presented in the same view (Summary window) helps find regions suitable for primer design. Turning on the Matching Bases/Residues as Dashes quickly shows regions of homology and divergence. I design primers to either or both depending on the project.
Dean Regier, Research Scientist DNA core laboratories