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09/16/2022

Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Phillipines 2012

All laboratory activities involving the virus isolates was conducted following BSL-2 biosafety practices and procedures in BSL-2 laboratory. Viral RNA was extracted and screened for the CHIKV by using Reverse-transcription PCR (RT-PCR) previously described6. The E1 (N = 19) genes of positive sample were subsequently sequenced and analyzed to determine the virus genotype. Isolates identified as Asian genotype (N = 18) were subjected tofull genome sequencing using the Ion Torrent sequencing platform (Life Technologies, USA). The raw sequence reads were assembled using the GSNAP algorithms as implemented in SequencherV5.2.2.

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Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Phillipines 2012

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Independent Emergence of the Cosmopolitan Asian Chikungunya Virus, Phillipines 2012

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