Q: Can I get a copy of Sequencher for evaluation before I buy?
Yes. You can get a free 3 day viewer demo by downloading and installing Sequencher, launching, and selecting "Try Viewer Now". You can also get a free 30 day full evaluation copy by downloading and installing Sequencher, launching, and selecting "Get Evaluation Copy" to get the process started. Both the Viewer and Evaluation versions have all the latest features and you can try them with your own sequence data.
Q: What is the current version of Sequencher?
The current release of Sequencher and Forensic-enabled Sequencher is version 5.2.3. If you are currently using an older version and would like to upgrade, please contact us.
Q: Does Sequencher work with 64 bit processors?
In general, the answer is yes. Sequencher 4.10.1 and later versions will automatically install 64 bit drivers on 64 bit operating systems. Versions of Sequencher prior to 4.10.1 will not install any drivers on 64 bit operating systems. Please download and install the 64 bit compatible drivers.
Hardware - Windows Users
Q: What are the computer hardware requirements to run Sequencher on Windows?
The minimal requirements to run Sequencher on Windows is 150MB of free hard disk space and 512MB of memory.
Q: What versions of the Windows Operating System does Sequencher support?
Sequencher 4.8 and higher are supported on Windows 8.1, Windows 8, Windows 7, Vista, and XP.
Hardware - Mac Users
Q: What are the computer hardware requirements to run Sequencher on Mac?
The minimal requirements to run Sequencher on Mac is 225MB of free hard disk space and 512MB of memory.
Q: What versions of Mac does Sequencher support?
The current version of Sequencher, version 5.2.3, is supported on the following Mac platforms: 10.5, 10.6, 10.7, 10.8, and 10.9.
Q: Does the Sequencher Server (Network Tools) run on Mac OS 10.9 (Mavericks)?
Yes, the current Sequencher Server release supports OS 10.5 through 10.9 and will serve Sequencher 5.2 and newer. It will not serve a license to older versions of Sequencher. The legacy Sequencher Server, 6.2.1 (which requires a hardware key), is available to support older versions of Sequencher and will install and run on Mac OS 10.4 through 10.8. You can find this version on our legacy support page.
Q: Can I run Sequencher on a Power PC processor?
PowerPC was a microprocessor used in Apple Macintosh computers between 1944 and 2006. Sequencher supported the PowerPC architecture until Sequencher version 5.0. From version 5.0.1 until now, Sequencher runs on the Intel processor based Macs.
Q: What about Network Licensing? How many computers can I install it on?
Gene Codes does not limit your number of installations, only the number of concurrent users, and all of your users will receive technical support.
Q: Does a Sequencher license expire?
Sequencher licenses are perpetual, so you can always use the latest version of Sequencher that you are licensed for. In addition, you will recieve upgrades to the latest version of Sequencher for as long as you are a member of the maintenance program.
For short-term projects or if you have a need for surge capacity, then 6 month and 12 month project licenses are available.
Q: Can I change from a standalone license to a network license (or vice-versa)?
Yes, you can always upgrade a standalone license to a network license or revert your network license to a standalone at anytime.
Q: What is a soft license?
A soft license doesn’t require having a hardware key attached to your computer. Instead, Sequencher uses a license file located on your computer to validate it’s a licensed copy.A soft license doesn’t require having a hardware key attached to your computer. Instead, Sequencher uses a license file located on your computer to validate it’s a licensed copy.
Q: What is an activation key and how do I get one?
An activation key is a unique 16 character key that encodes the details of your purchased license. An activation key is sent to you when you purchase Sequencher. Once an activation key has been created, it is used to provide your customized license for validating your purchased copy of Sequencher.
Q: How long will it take to get my license?
Once you’ve sent your license information to Gene Codes at ActivateMe@genecodes.com, you should receive your license by the next business day based on the US calendar.
Q: How do I get a standalone license and how does it get installed?
Launch Sequencher on the computer upon which you wish to run. The License Sequencher dialog will open. If you have purchased a new license, then you should have received a 16 digit Activation Key. Paste or type it into the Enter Activation Key field and click on the New License button. If instead, you’re upgrading an older version of Sequencher, then you don’t need an Activation Key. Just make sure that your Sequencher hardware key is plugged in and click on the Upgrade License button. You’ll need to decide whether or not you want the new license file to be tied to the hardware key or to this computer.
You’ll be prompted to save a SequencherLicenseRequest file. Please email that file to us at ActivateMe@genecodes.com. We’ll email your license file back to you.
Once you have a license file, launch Sequencher and, in the License Sequencher dialog, Browse to the file, select it, and click on the Open button. Clicking Install will install a copy of the license file for Sequencher.
Q: What types of licenses are available for Sequencher 5.2 and later versions?
Standalone license – locked/tied to a particular hardware key or computer.
Network licenses – licenses that are served by the Sequencher License Server which controls the number of concurrent users who may run Sequencher. Users who wish to access a network license for Sequencher install a client license on their workstation.
Both Standalone and Network licenses are available as perpetual or time-limited licenses:
- Perpetual licenses – these perpetual licenses give users full functionality for the purchased version and do not expire.
- Project licenses – these time-limited licenses give users full functionality but expire after the purchased number of days.
Fully-functional Evaluation licenses – there is a free 30-day option available for you to try Sequencher with your own data.
Q: Can Sequencher run on a Virtual Machine?
You cannot use a virtual machine as your Sequencher License Server and you cannot use a standalone license on a virtual machine. However, you can run Sequencher on a virtual machine provided you are running as a client of a Sequencher Server and have a client license file installed.
Q: I want to use Sequencher in the office and on my laptop computer outside of the office (at home, or when I travel, etc.). I don't have a hardware key anymore so what do I do?
In the absence of a hardware key, a standalone license must be tied to a single computer. If you want to run on multiple computers, you’ll need to purchase a license for each individual computer. Installing your license on a mobile computer allows you to use Sequencher on the go.
If your institution allows you to connect to the internal network, you have more options. You may run Sequencher remotely through VPN or remote desktop. Or you may purchase a network license of Sequencher, connecting from client workstations both at work and away. Your local system administrator can tell you if these options are available to you.
Q: Why is Sequencher in Viewer Mode?
Sequencher will enter Viewer Mode (no printing, saving, copying, exporting, reporting) if you are trying a Viewer Demo.
Otherwise, it’s possible to lose your license and drop into Viewer Mode.
If you have a standalone license and your license is tied to a USB hardware key and Sequencher has lost communication with the key, then you’ll be switched to Viewer Mode. Check to make sure that the key is still plugged in and lit. Try restarting Sequencher.
If you have a network license and either your license was revoked by an administrator or the Sequencher Server shut down or lost network communication with your workstation, rather than quit, Sequencher switched to Viewer Mode. Check with your server administrator to inquire about your license issue.
If there is still a problem validating your license, please contact Gene Codes Technical Support.
Q: Will KeyServer serve 5.2 licenses?
Starting with Sequencher 5.2, KeyServer is no longer supported. Instead, licensing is managed by a Sequencher Server using license files on the server computer itself and client license files on all computers running Sequencher.
Q: How do I set up a Sequencher Server for Sequencher 5.2 and later versions?
You need to install the server software and a license file. See the Sequencher Server Guide at http://genecodes.com/support/sequencher-server-download for instructions on how to set up a Sequencher Server and get a license file to install. The Sequencher Server cannot be set up or run on a virtual machine.
Q: How should I choose a Sequencher License Server?
The network requires that you designate one computer as your server. The server may be any Macintosh or Windows based computer in your network, but it is best if it has a static IP address or DNS name and is left on at all times.
Q: When I’m in the License Tools application and click on the ‘Activateme@genecodes.com’ link at the bottom of the dialog, nothing happens. What should happen?
When you click on the link, an email client window should pop up with the To, Subject, and Body all filled in with information Gene Codes needs in order to create a software license for you. You’ll need to have a default mail client set up on your computer with the “mailto” functionality enabled in order for this email process to work. Once the email is generated as a result of clicking on the link, just send it on.
If your computer does not have an email account established (for example, it is a shared lab computer), then copy the contents of the license data using the “Copy to Clipboard” button and paste the information into a text document. Move that document to your email computer and send it as an attachment to ActivateMe@genecodes.com.
Q: How do I get a client license in order to run Sequencher from a Sequencher Server (NW license)?
Contact your license administrator for help in getting a client license file. Once you have a license file, launch Sequencher and, in the License Sequencher dialog, Browse to the file, select it, and click on the Open button. Clicking Install will install a copy of the license file for Sequencher. If the license is valid, and your License Server is up and running, Sequencher will launch. For instructions on installing server licenses, follow the instructions in the Sequencher Server Guide.
Maintenance and Support
Q: How do I get technical support for Sequencher?
Unlimited Sequencher technical support is available to all current members of the Maintenance Program by email and by phone. We encourage you to phone us, but if you prefer email, please complete the form on the Request Tech Support page, describing your problem as completely as possible. Include your full contact information and your registration number, or the original purchaser's name.
We also have a troubleshooting guide as part of this FAQ that could answer many of your questions as well.
Q: How do I find my Registration Number?
Your registration number will be displayed when you select About Sequencher from the Help menu (the Sequencher menu on Mac).
Q: What is the Maintenance Program?
Gene Codes maintenance plan for Sequencher is an annual plan that provides you with valuable benefits such as unlimited technical support, and a guarantee of at least one upgrade. When you are covered under the maintenance plan, there are no charges for overnight shipping of replacement CDs, technical consulting for sequencing projects, or rearrangement of your network configuration.
When you purchase Sequencher, you receive the first year's maintenance free of charge.
Q: When I start Sequencher 5.2 or later, I get a License Sequencher dialog. Why?
You need a valid Sequencher license in order to launch Sequencher. If you see this dialog, it could be due to one of the following reasons.
- No license exists in the expected location. For information on activating Sequencher, see the FAQ “How do I get a standalone license and how does it get installed? ”
- The license is not compatible with the Sequencher product you are using. For example, your current license has expired or is for a different version of Sequencher.
You can contact Gene Codes support at firstname.lastname@example.org for assistance.
Q: I moved my Sequencher hardware key to a different computer but get the License Sequencher dialog when I launch Sequencher. What’s wrong?
If you have Sequencher 5.2 or later and you received a license that is locked to the hardware key, you must also install the license file as well as move the key to the new computer. For information on activating Sequencher using your license file, see the FAQ “How do I get a standalone license and how does it get installed?”
Q: When I select the ‘Try Viewer Now’ button, why do I get the message ‘Sequencher could not obtain a valid license.‘?
You could get this message if you have already tried the Viewer or if you are running on a Virtual Machine.
When I select the ‘Try Viewer Now’ button, why do I get the message ‘Sequencher could not obtain a valid license.‘?
You could get this message if you have already tried the Viewer or if you are running on a Virtual Machine.
Q: When I launch Sequencher, why do I get the message ‘Sequencher could not obtain a valid license.‘?
You could get this message for a number of reasons. The 2ndpart of the message will provide additional information on the particular cause, like ‘License has expired’, ‘Wrong host for license’, ‘Unlicensed platform’, etc.
Q: My license didn't arrive/I lost my license, can you send another?
Certainly, we’ll be glad to send you another copy of your original license file or help determine what happened to the file. Simply contact Gene Codes Technical Support or your Sales representative and explain the situation. We’ll take it from there.
Q: I just got a new computer and now my license doesn't work, what can I do?
If you have a standalone license that was locked to your old computer, you’ll need to get a new license file that’s based on the machine configuration of your new computer. Please contact Gene Codes Technical Support at email@example.com for help with this.
If you have a standalone license that is locked to a USB hardware key, you’ll need to attach the hardware key and install the license file onto the new computer.
If your site has a network version of Sequencher, you’ll need a SequencherClient.lic file on your new computer in order to obtain a license. Contact your System or License Administrator to get help with obtaining a client license file. Then, launch Sequencher, Browse to and select the license file, and then Install it.
Q: Why am I prompted for a password when I install Sequencher on my Macintosh?
The prompt is from your Macintosh operating system. When you install an application on OSX the operating system insures that you have administrative privileges by requiring a password.
Q: My Windows version of Sequencher doesn't recognize the projects that I created on my Mac version. What do I do?
Windows applications are more dependent on extensions than Mac applications. In order to open Sequencher projects that originate on a Mac, add a .SPF extension to the file if one does not already exist.
Q: Some of my fonts do not look right on Japanese/Chinese/Korean versions of Windows 8, Windows 7, Vista, or XP. What should I do?
Some versions of Windows have problems with two-byte language systems and Microsoft has provided a fix to that problem. Download a new ASIFONT.map file, here: asifont.zip then, follow these simple steps:
- Make sure Sequencher is not running.
- Unzip the asifont.zip file.
- Go to your '<drive>\Program Files\Gene Codes\Sequencher[VERSION]' folder.
- Rename asifont.map that you find there as 'old asifont.map'.
- Copy the newly unzipped asifont.map file into that same folder.
- Relaunch Sequencher.
Q: How do I add restriction enzymes?
If you wish to use a restriction enzyme that is not in the default set of enzymes supplied with Sequencher, open a project and highlight the fragments or contigs for which you wish to specify a new enzyme. Select the Specify Restriction Enzymes option from the Window menu and check the Allow changes checkbox. You can then use the New Enzyme button to create a new enzyme record. Click the Done button when you are finished.
Q: Does Sequencher import Vector NTI files?
Yes. Starting with Sequencher version 4.9, MA4 files with all of their associated features can be imported. Starting with Sequencher 4.10.1, Contig Express files can also be imported along with their associated features, quality scores, and trace data.
Q: How do I import features from a GenBank sequence into Sequencher?
Sequencher imports all GenBank features with a defined range, including joined features, as editable features in Sequencher. To import any individual or concatenated sequence into Sequencher, use the IMPORT > SEQUENCES command.
Q: How do I share Sequencher Windows and Sequencher Macintosh projects?
Project files created by version 4.1 and higher of Sequencher (Mac or Windows) are entirely cross-platform compatible. Additionally, Sequencher is backwards compatible with version 4.0 project file formats on the same platform (any Mac version of Sequencher can read project files created by a previous version of Sequencher on the Mac, and the same is true for Windows). It is therefore possible to read an old project file with a new version of Sequencher and then save it in the current cross-platform format.
Sequencher project files created on the Macintosh by version 3.1 of Sequencher are not cross-platform compatible. You can download a Mac-to-PC converter (Macintosh Classic version only). However, versions of Sequencher starting with 5.0.1 can not open 3.x project files. The message 'The file "<project>.SPF" is not a valid Sequencher project. Try opening another project.' will be displayed.
Q: Where can I find some example scripts to run Sequencher using AppleScript commands?
We have prepared some sample scripts to help you get started using AppleScript with Sequencher. Click here to download a zip file with several examples. If you develop scripts of your own that you'd like to share with the community, we'd be happy to pass them on. Contact us at firstname.lastname@example.org.
Legacy Support for Sequencher 5.1 and Earlier
Q: I run my KeyServer on a Windows 8, Windows 7, Vista, or XP SP2 machine. I can run my client from the Server machine, but I am unable to log onto the Server from other clients. What do I do?
The firewall in Windows 8, Windows 7, Vista, and XP Service Pack 2 will not allow a remote client to log onto the server unless you open specific UDP ports. Below are two links which document how to get to the Windows Firewall administration panel and how to open ports. The two ports that need to be opened are UDP 19283 and UDP 19315.
Q: When I start Sequencher, I see this message: Sequencher was unable to find a key. The program will run only in Demo Mode. What do I do?
This error message is possible for both standalone and network versions of Sequencher Licenses. Although the underlying causes differ greatly between the two license types, the error triggers because Sequencher is unable to obtain a license from either a hardware key attached to the machine running the program, or from a Sequencher License Server or KeyServer network.
If you have a key attached to your computer…
Determine if your key is a standalone or network key. The number of users for network licenses is printed on the side of your key. If your key defines a number of users, then it is a network key.
If you are using a network key…
When you obtain a Sequencher license from a network key, you must log on to KeyServer using KeyAccess software, even if the network key is attached to your machine. A lost connection to the server is the most likely reason for triggering the demo mode error with a network license. To reconnect, confirm the IP address of your KeyServer, and then login using KeyAccess.
If you are using a standalone key…
There are four reasons that Sequencher will prompt you with this message if you are using a standalone key: 1) You are using a Purple Eve 3 Macintosh standalone key; you must upgrade to the new SuperPro key (please contact Gene Codes sales department), 2) the key is broken, 3) the port is not configured correctly or compatible with your key (note Windows NT and earlier operating systems are not compatible with USB), 4) the installation did not include the driver for your key. To check if the key is broken, check to see that the light is on (USB only). If you have access to another standalone key, plug it in to see if that key enables your installation. Installing Sequencher on another machine is a good means of testing whether you have a configuration specific problem. If you don’t have the right drivers, try reinstalling Sequencher from your CD.
If you don’t have a key attached to your computer…
Then you must be using a network version of Sequencher. Follow the above recommendations for using a network key.
If none of the above solves your problem, please Contact Technical Support, or email email@example.com, quoting your registration number.
Q: I have updated my Windows Operating System to Windows Vista or later, and now Sequencher only launches in Demo Mode. What do I do?
The versions of Sequencher prior to 4.9 are not completely compatible with the Windows 8, Windows 7 or Vista. If you have Sequencher 4.8 or earlier and are running Windows 8, Windows 7 or Vista, please either upgrade to a more current version or install the latest sentinel drivers from SafeNet.
Q: How do I set up a Sassafrass Keyserver?
We provide detailed instructions, but here are the six basic steps to the installation process.
1. Choose a computer on your network to act as a Sequencher License Server.
2. Run the Sequencher License Installer on that computer to install KeyServer and all the files necessary to recognize Sequencher licenses.
3. Plug in the USB hardware key as the installer directs (it contains your licenses.
4. At the end of the installation process, the IP address of the Sequencher License Server computer will be displayed. Write it down. You'll need that address to set up each client computer to run Sequencher.
5. Install the client software, KeyAccess, on each of these computers by running the Sequencher Client/Setup.exe on Windows, or K2Client on OS X. You'll be asked to type in the address of the Sequencher License Server computer.
6. Run the Sequencher Installer and Restart.
If you have any difficulty with the installation process, please Contact Technical Support.
Q: What is Sequencher License Server? What is KeyServer? KeyServer is a license management application available from Sassafras Software. The network version of Sequencher includes a limited version of this software for managing Sequencher licenses on a net
KeyServer is a license management application available from Sassafras Software. The network version of Sequencher includes a limited version of this software for managing Sequencher licenses on a network.
Sequencher licenses can be served over TCP/IP, AppleTalk, or IPX. Sequencher for Windows will work on a Novell Server network (although not on the Novell server itself), but the Macintosh version is not supported in this environment.