"The most popular request I receive in the DNA core lab is: find the good clone among this group of 'candidate' minipreps. Alignment of ABI data files against a 'reference' sequence (template file) quickly eliminates defectives by showing not only base changes but the consequences to the protein translation.
The second most popular request is: validate this new maxiprep so it can be given to ... or used for ... or expressed in ... Rapid assembly and editing of total plasmid sequence and then comparison to the reference file on record is all done in Sequencher.
The third most popular request is: compare this library of (protein improvement) mutants and tell me which ones have protein changes. This is done by alignment in Sequencher and then formatting the Summary view to display the resulting changes in protein translation.
For my own projects, I use Sequencher as a very general multiple-alignment sequence editor. Most recently, I've been using it to make comparisons of genes retrieved from the databases for cross-species cDNA cloning. Showing regions of homology at the protein level and then having the colinear DNA sequence presented in the same view (Summary window) helps find regions suitable for primer design. Turning on the Matching Bases/Residues as Dashes quickly shows regions of homology and divergence. I design primers to either or both depending on the project.
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Sequencher Server Download
Sequencher License Server Downloads
Detailed information regarding setting up and using a Sequencher License Server Network may be found in thes Installation Instructions.
Sequencher License Server tools for Macintosh
Current Macintosh Sequencher License Server tools are compatible with 10.4 and above. This requires a green USB SuperPro key.
- Sequencher License Server 6.2.1 for Mac [DMG]
- Sequencher Client.dmg
- Mac Client Installation Instructions
Sequencher License Server tools for Windows
Current Windows Sequencher License Server tools are compatible with Windows XP and above. This server is compatible with green and red SuperPro keys.
Note: You must first extract the installer from ZIP files to your computer and then run the Setup.exe program.
- Sequencher License Server 6.2.1 for Windows [zip]
- Sequencher Client 6.2.1 for Windows [zip]
- Win Client Installation Instructions
For support for older operating systems, please go to the Legacy Support Page.


